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81.
Orf virus (ORFV) causes contagious skin disease that mainly affects sheep and goats with zoonotic potential. However, there is not enough information about the association between ORFV and occurrence of skin disease in cattle. The present study describes outbreaks of ORFV infection in cattle in different provinces that are located in the Aegean, Central Anatolian and Mediterranean regions of Turkey. During the months of June and August 2017, vesicular fluid and scab samples were collected from cattle which had proliferative skin lesions. First, presence of lumpy skin disease virus (LSDV) and bovine herpesvirus 2 (BoHV-2, known as the causative agent of pseudo-lumpy skin disease) were investigated by real time PCR and PCR, respectively. Then, samples tested for the presence of parapoxviruses by PCR using primers specific to major envelope protein gene (B2L). Parapoxvirus DNA was detected in investigated samples whereas LSDV and BoHV-2 DNA were not detected. The analysis of the B2L gene sequences revealed that cattle were infected with ORFV. The isolates in the present study shared 100% sequence identity at the nucleotide and amino acid level when compared with previously characterised Turkish field ORFV isolates from goats in 2016. Results of the study show unusual infection of cattle with ORFV, and suggest that ORFV jumps the host species barrier from goats to cattle. 相似文献
82.
The objective of this study was to determine the prevalence of intraoperative electrolyte disturbances and risk factors associated with perianesthetic death in horses undergoing colic surgery. The files of 120 horses meeting the inclusion criteria were reviewed. Data retrieved from the medical records, including demographic data and the occurrence of electrolyte disturbances and other intraoperative complications, were analyzed with a two step-approach by univariate and multivariate logistic regression models. Hypocalcemia was the most represented electrolyte disturbance (52.5%), followed by hypokalemia (30.0%) that was associated with intraoperative administration of salbutamol (P = .045). Perianesthetic death occurred in 46 horses, accounting for an overall mortality rate of 38.3%. Risk factors associated with death were anesthetic duration (P = .001), body weight (P = .020), presence of gastric reflux before anesthesia (P = .021), and intraoperative tachycardia (P = .043) and acidosis (P = .025). The mortality in the study population was comparable to previously reported findings. Based on the study findings, it is advisable to optimize hemodynamics prior to anesthesia, in order to prevent intraoperative tachycardia that is associated with increased risk of death. Heavier horses and those with gastric reflux may have a higher risk of fatalities, and intraoperative salbutamol administration may contribute to hypokalemia. 相似文献
83.
Nonstructural carbohydrates of pasture plants, comprising water-soluble carbohydrates (WSCs) and starch, may contribute to excessive consumption of rapidly fermentable carbohydrates by grazing horses. Seasonal and diurnal variation in WSCs were studied in red (Trifolium pratense L.) and white clovers (Trifolium repens L.) subjected to a typical management regime of rotationally grazed horse pastures. Two red and two white clover cultivars from monoculture plots were harvested after 4 weeks of growth from April to October of 2015, in the morning and afternoon of each harvest date. Water-soluble carbohydrates were quantified for each harvest, and starch was quantified for two harvests. Mean monthly WSC concentrations ranged from 80 to 99 mg/g (freeze-dried weight basis), whereas mean starch concentrations were 31 and 40 mg/g. In September, white clover had 14% more WSCs than red clover (P < .0001). Water-soluble carbohydrate concentrations were 10% higher in the afternoon than in the morning (P < .0001). Starch concentrations were 290% higher in the afternoon than in the morning (P < .0001), and nonstructural carbohydrate concentrations in the afternoon averaged 150 mg/g. Further studies are needed to determine whether the mixed grass-legume pastures of central Kentucky accumulate enough nonstructural carbohydrates to present risk factors for equine metabolic or digestive dysfunction. 相似文献
84.
Equine obesity is increasing in prevalence, and weight loss diets are frequently recommended for these horses. However, there are also management situations in which horses are deemed to be too thin. To monitor the efficacy of weight change programs, estimates of body fat are often made. There are several systems available to estimate body fat, and there are benefits and challenges to using each method. The objective of this study was to compare four different methods of estimating body fat in Thoroughbred horses. In 14 mature Thoroughbred horses, relationships among body condition score (BCS), morphometric measurements, ultrasonic measures of subcutaneous fat depots, and estimation of total body fat (BFD) via measurement of total body water through deuterium oxide dilution were evaluated. Body condition scores ranged from 4.5 to 6.5 on a 9-point scale. Body condition score, heart girth-to-body weight ratio, and BFD were all positively correlated with each other (P < .05). Subcutaneous fat depth at the tailhead tended to be positively related to BFD when only horses with BCS ≥ 5 were included (P = .0680). These data suggest that BCS remains a simple means of monitoring adiposity in mature horses in moderate condition. Tailhead fat depots may become useful for monitoring changes in body fat in Thoroughbreds with a BCS above 5, although more work with animals of higher adiposity is required and at different times of year. 相似文献
85.
Cortisol is involved in the initiation of parturition and we hypothesized that increased maternal cortisol release advances the onset of foaling. Transport is a stressor for horses and induces an increase in cortisol release. To determine stress effects on the time of foaling, late-pregnant mares were transported by road for 3 hours (n = 12) or remained in their stable as controls (n = 4). Starting on day 325 of gestation, saliva and blood samples were taken for cortisol and progestin analysis, respectively. Fetomaternal electrocardiograms were recorded repeatedly. Mares were checked for impending parturition and changes in precolostrum pH. When pH decreased to 6.5, mares were either transported or left untreated. After birth, saliva was collected repeatedly from mares and their foals and heart rate (HR) was recorded. Foals were checked for maturity and health. Gestation length was 337 ± 2 days in stressed and 336 ± 2 days in control mares. Cortisol concentration increased from 3.3 ± 0.9 to 8.4 ± 0.8 ng/mL in transported mares (P < .001) and remained constant in controls. Maternal HR and heart rate variability (HRV) did not differ between groups and neither fetal HR nor HRV changed in response to transport. In transported mares, time from precolostrum decrease to parturition was shorter (40 ± 10 hours) than the respective time in controls (134 ± 49 hours, P < .01). Neither duration of foaling nor times to first standing and suckling of foals or the postnatum increase in HR and decrease in HRV differed between groups. In conclusion, transport-induced maternal cortisol release may have advanced the onset of foaling. 相似文献
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